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FastSCRIPT™ cDNA Synthesis Kit
  • FastSCRIPT™ cDNA Synthesis Kit

FastSCRIPT™ cDNA Synthesis Kit

Cat No. Size Price Quantity
31-5300-0025R 25 rxns $128.75
31-5300-0100R 100 rxns $432.00

Description

The FastSCRIPT™ cDNA Synthesis Kit contains everything you need to generate unbiased, efficient, sensitive and high-quality cDNA for your downstream experiments. Our kit removes the need for user optimization, making it an ideal solution due to its robust performance and ease of use. The kit contains a proprietary M-MLV Reverse Transcriptase (RT) molecule blended with an optimized RNase inhibitor, a mix that is both thermostable and extremely active. This RTase mix synthesizes cDNA at a temperature range of 45–55°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases. The relative concentrations of random hexamers and anchored oligo(dT) primers have been designed specifically to generate cDNA optimized for use in Real-Time PCR experiments. The FastSCRIPT cDNA Synthesis Kit can be used with total or poly(A)+ RNA, miRNA and viral RNA targets and provides sensitive detection of low copy number transcripts.

 

Recent Citations: 

Murphy S, Patrick K, Thoner T, Edwards RW and Gubbels Bupp MR. 2016. T cell up-regulation of CD127 is associated with reductions in the homeostatic set point of the peripheral Tcell pool during malnourishment. Biochemistry and Biophysics Reports. doi:10.1016/j.bbrep.2016.06.006

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The FastSCRIPT™ cDNA Synthesis Kit contains everything you need to generate unbiased, efficient, sensitive and high-quality cDNA for your downstream experiments. Our kit removes the need for user optimization, making it an ideal solution due to its robust performance and ease of use. The kit contains a proprietary M-MLV Reverse Transcriptase (RT) molecule blended with an optimized RNase inhibitor, a mix that is both thermostable and extremely active. This RTase mix synthesizes cDNA at a temperature range of 45–55°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases. The relative concentrations of random hexamers and anchored oligo(dT) primers have been designed specifically to generate cDNA optimized for use in Real-Time PCR experiments. The FastSCRIPT cDNA Synthesis Kit can be used with total or poly(A)+ RNA, miRNA and viral RNA targets and provides sensitive detection of low copy number transcripts.

 

Recent Citations: 

Murphy S, Patrick K, Thoner T, Edwards RW and Gubbels Bupp MR. 2016. T cell up-regulation of CD127 is associated with reductions in the homeostatic set point of the peripheral Tcell pool during malnourishment. Biochemistry and Biophysics Reports. doi:10.1016/j.bbrep.2016.06.006

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Product Details

Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.