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CYBRFast™ 1-Step RT-qPCR Lo-ROX Kit
  • CYBRFast™ 1-Step RT-qPCR Lo-ROX Kit

CYBRFast™ 1-Step RT-qPCR Lo-ROX Kit

Cat No. Size Price Quantity
31-5201-0100R 100 rxns $105.00
31-5201-0300R 300 rxns $285.00

Description

CYBRFast™ 1-Step RT-qPCR Lo-ROX Kit contains everything required for efficient cDNA synthesis and qPCR in a single tube using real-time instruments that support normalization with a standard ROX passive reference dye at a final concentration of ≥ 50 nM. This approach offers simplicity and convenience and minimizes the possibility for contamination. Tonbo’s proprietary CYBRFast™ GREEN DNA intercalating dye does not inhibit PCR, resulting in better fluorescent signals than other dyes, and is optimized for use with CYBRFast™ Hot Start Taq DNA Polymerase. Novel hot start technology prevents primer dimer formation and non-specific amplification resulting in higher yields and specificity. 1-Step Kits include a proprietary M-MLV Reverse Transcriptase (RT) molecule blended with an optimized RNase inhibitor, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases. Our M-MLV RT is ultra-stable and highly active, working on varied and difficult templates. Convenient and reliable, Tonbo’s CYBRFast 1-Step RT-qPCR Lo-ROX Kit is ideal for the sensitive and efficient quantification of RNA templates, including low copy number targets, under standard or fast cycling conditions. Please refer to our Instrument Compatibility Guide to ensure you have the correct ROX concentration for your system.
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CYBRFast™ 1-Step RT-qPCR Lo-ROX Kit contains everything required for efficient cDNA synthesis and qPCR in a single tube using real-time instruments that support normalization with a standard ROX passive reference dye at a final concentration of ≥ 50 nM. This approach offers simplicity and convenience and minimizes the possibility for contamination. Tonbo’s proprietary CYBRFast™ GREEN DNA intercalating dye does not inhibit PCR, resulting in better fluorescent signals than other dyes, and is optimized for use with CYBRFast™ Hot Start Taq DNA Polymerase. Novel hot start technology prevents primer dimer formation and non-specific amplification resulting in higher yields and specificity. 1-Step Kits include a proprietary M-MLV Reverse Transcriptase (RT) molecule blended with an optimized RNase inhibitor, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases. Our M-MLV RT is ultra-stable and highly active, working on varied and difficult templates. Convenient and reliable, Tonbo’s CYBRFast 1-Step RT-qPCR Lo-ROX Kit is ideal for the sensitive and efficient quantification of RNA templates, including low copy number targets, under standard or fast cycling conditions. Please refer to our Instrument Compatibility Guide to ensure you have the correct ROX concentration for your system.
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Product Details

Name CYBRFast™ 1-Step RT-qPCR Lo-ROX Kit
Cat. No. 31-5201
Citations

Ramos Muniz MG, Palfreeman M, Setzu N, Sanchez MA, Saenz Portillo P, Garza KM, Gosselink KL, Spencer CT. Obesity Exacerbates the Cytokine Storm Elicited by Francisella tularensis Infection of Females and Is Associated with Increased Mortality. Biomed Res Int. 2018 Jun 26;2018:3412732. doi: 10.1155/2018/3412732

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Manan M. Mehta, Samuel E. Weinberg, Elizabeth M. Steinert, Krishan Chhiba, Carlos Alberto Martinez, Peng Gao, Harris R. Perlman, Paul Bryce, Nissim Hay, and Navdeep S. Chandel. Hexokinase 2 is dispensable for T cell-dependent immunity. Cancer Metab. 2018 Aug 17;6:10. doi: 10.1186/s40170-018-0184-5

» View on PubMed

Application PCR
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.