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CFSE
  • CFSE

CFSE

Cat No. Size Price Quantity
13-0850-U500 500 µg $23.00

Description

CFSE is a non-fluorescent molecule that easily diffuses across cell membranes. Inside the cell, acetate groups are cleaved by intracellular esterases yielding a fluorescent molecule whose succinimidyl ester group covalently interacts with primary amines of intracellular proteins. CFSE is compatible with standard intracellular staining protocols using aldehyde fixation and saponin-based permeabilization. CFSE is widely used to track cell division and to monitor cell migration in vivo. CFSE can be detected using standard fluorescein filter sets by fluorescence microscopy or flow cytometry.

CFSE Labeling ProtocolDownload the CFSE Labeling Protocol.

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CFSE is a non-fluorescent molecule that easily diffuses across cell membranes. Inside the cell, acetate groups are cleaved by intracellular esterases yielding a fluorescent molecule whose succinimidyl ester group covalently interacts with primary amines of intracellular proteins. CFSE is compatible with standard intracellular staining protocols using aldehyde fixation and saponin-based permeabilization. CFSE is widely used to track cell division and to monitor cell migration in vivo. CFSE can be detected using standard fluorescein filter sets by fluorescence microscopy or flow cytometry.

CFSE Labeling ProtocolDownload the CFSE Labeling Protocol.

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Product Details

Name CFSE
Cat. No. 13-0850
Protocol CFSE Labeling Protocol
Excitation Laser Blue (488 nm)
Emission (nm) 521
Formulation lyophilized (500 ug)
Application Flow Cytometry
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.