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CFSE
  • CFSE

CFSE

Cat No. Size Price Quantity
13-0850-U500 500 µg $23.00

Description

CFSE is a non-fluorescent molecule that easily diffuses across cell membranes. Inside the cell, acetate groups are cleaved by intracellular esterases yielding a fluorescent molecule whose succinimidyl ester group covalently interacts with primary amines of intracellular proteins. CFSE is compatible with standard intracellular staining protocols using aldehyde fixation and saponin-based permeabilization. CFSE is widely used to track cell division and to monitor cell migration in vivo. CFSE can be detected using standard fluorescein filter sets by fluorescence microscopy or flow cytometry.

CFSE Labeling ProtocolDownload the CFSE Labeling Protocol.


Recent Publications:

Do P, Perdue LA, Chyong A, Hunter R, Dougan J, Henry CJ, Porter CC, Dreaden EC. Rapid Assembly and Screening of Multivalent Immune Cell-Redirecting Therapies for Leukemia. ACS Comb Sci. 2020 Oct 12;22(10):533-541. doi: 10.1021/acscombsci.0c00081. Epub 2020 Aug 18. PMID: 32786324.

» View on PubMed

Domínguez-Romero AN, Martínez-Cortés F, Munguía ME, Odales J, Gevorkian G, Manoutcharian K. Generation of multiepitope cancer vaccines based on large combinatorial libraries of survivin-derived mutant epitopes [published online ahead of print, 2020 Jul 3]. Immunology. 2020;10.1111/imm.13233. doi:10.1111/imm.13233.

» View on PubMed

Brincks E. L., Adams J., Wang L., Turner B., Marcinowicz A., Ke J., Essmann M., Mautino L. M., Allen C. Van, Kumar S., Vahanian N., Link C., Mautino M. R. et al Indoximod opposes the immunosuppressive effects mediated by IDO and TDO via modulation of AhR function and activation of mTORC1. Oncotarget. 2020; 11: 2438-2461.

View on Oncotarget

Rana PS, Mudrak NJ, Lopez R, Lynn M, Kershner L and Model MA. 2017. Phase separation in necrotic cells. Biochem Biophys Res Commun. doi: 10.1016/j.bbrc.2017.08.123.

» View on PubMed

Murphy S, Patrick K, Thoner T, Edwards RW and Gubbels Bupp MR. 2016. T cell up-regulation of CD127 is associated with reductions in the homeostatic set point of the peripheral Tcell pool during malnourishment. Biochemistry and Biophysics Reports. doi:10.1016/j.bbrep.2016.06.006

» View on PubMed

Barwick BG, Scharer CD, Bally APR and Boss JM. 2016. Plasma Cell Differentiation is Coupled to Division-Dependent DNA Hypomethylation and Gene Regulation. Nat Immunol. 17(10):1216-25

» View on PubMed

View More

CFSE is a non-fluorescent molecule that easily diffuses across cell membranes. Inside the cell, acetate groups are cleaved by intracellular esterases yielding a fluorescent molecule whose succinimidyl ester group covalently interacts with primary amines of intracellular proteins. CFSE is compatible with standard intracellular staining protocols using aldehyde fixation and saponin-based permeabilization. CFSE is widely used to track cell division and to monitor cell migration in vivo. CFSE can be detected using standard fluorescein filter sets by fluorescence microscopy or flow cytometry.

CFSE Labeling ProtocolDownload the CFSE Labeling Protocol.


Recent Publications:

Do P, Perdue LA, Chyong A, Hunter R, Dougan J, Henry CJ, Porter CC, Dreaden EC. Rapid Assembly and Screening of Multivalent Immune Cell-Redirecting Therapies for Leukemia. ACS Comb Sci. 2020 Oct 12;22(10):533-541. doi: 10.1021/acscombsci.0c00081. Epub 2020 Aug 18. PMID: 32786324.

» View on PubMed

Domínguez-Romero AN, Martínez-Cortés F, Munguía ME, Odales J, Gevorkian G, Manoutcharian K. Generation of multiepitope cancer vaccines based on large combinatorial libraries of survivin-derived mutant epitopes [published online ahead of print, 2020 Jul 3]. Immunology. 2020;10.1111/imm.13233. doi:10.1111/imm.13233.

» View on PubMed

Brincks E. L., Adams J., Wang L., Turner B., Marcinowicz A., Ke J., Essmann M., Mautino L. M., Allen C. Van, Kumar S., Vahanian N., Link C., Mautino M. R. et al Indoximod opposes the immunosuppressive effects mediated by IDO and TDO via modulation of AhR function and activation of mTORC1. Oncotarget. 2020; 11: 2438-2461.

View on Oncotarget

Rana PS, Mudrak NJ, Lopez R, Lynn M, Kershner L and Model MA. 2017. Phase separation in necrotic cells. Biochem Biophys Res Commun. doi: 10.1016/j.bbrc.2017.08.123.

» View on PubMed

Murphy S, Patrick K, Thoner T, Edwards RW and Gubbels Bupp MR. 2016. T cell up-regulation of CD127 is associated with reductions in the homeostatic set point of the peripheral Tcell pool during malnourishment. Biochemistry and Biophysics Reports. doi:10.1016/j.bbrep.2016.06.006

» View on PubMed

Barwick BG, Scharer CD, Bally APR and Boss JM. 2016. Plasma Cell Differentiation is Coupled to Division-Dependent DNA Hypomethylation and Gene Regulation. Nat Immunol. 17(10):1216-25

» View on PubMed

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Product Details

Name CFSE
Cat. No. 13-0850
Protocol CFSE Labeling Protocol
Excitation Laser Blue (488 nm)
Emission (nm) 521
Formulation lyophilized (500 ug)
Application Flow Cytometry
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.