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C57Bl/6 splenocytes were stimulated with 1X Cell Stimulation Cocktail (500X) (TNB-4975-UL100) (right panel) or unstimulated (left panel) and then stained with APC Anti-Mouse CD3e (20-0031), followed by intracellular staining with PE Anti-Mouse IL-2 (50-7021).
  • C57Bl/6 splenocytes were stimulated with 1X Cell Stimulation Cocktail (500X) (TNB-4975-UL100) (right panel) or unstimulated (left panel) and then stained with APC Anti-Mouse CD3e (20-0031), followed by intracellular staining with PE Anti-Mouse IL-2 (50-7021).

Cell Stimulation Cocktail (500X)

Cat No. Size Price Quantity
TNB-4975-UL100 100 µL $59.00
TNB-4975-UL400 400 µL (4 x 100 µL) $177.00

Description

Cell Stimulation Cocktail (500X) is a ready to use cocktail that can be used for polyclonal cell activation. It is composed of the phorbol ester, PMA (Phorbol 12-Myristate 13-Acetate), a calcium ionophore (Ionomycin), and the protein transport inhibitors Brefeldin A and Monensin. This Cell Stimulation Cocktail (500X) can be used to elicit cytokine production and retention in a variety of cell types allowing for detection of intracellular protein by flow cytometry.


Cell Stimulation for Cytokine Production ProtocolDownload the Cell Stimulation for Cytokine Production Protocol.

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Cell Stimulation Cocktail (500X) is a ready to use cocktail that can be used for polyclonal cell activation. It is composed of the phorbol ester, PMA (Phorbol 12-Myristate 13-Acetate), a calcium ionophore (Ionomycin), and the protein transport inhibitors Brefeldin A and Monensin. This Cell Stimulation Cocktail (500X) can be used to elicit cytokine production and retention in a variety of cell types allowing for detection of intracellular protein by flow cytometry.


Cell Stimulation for Cytokine Production ProtocolDownload the Cell Stimulation for Cytokine Production Protocol.

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Product Details

Name Cell Stimulation Cocktail (500X)
Cat. No. TNB-4975
Protocol Cell Stimulation for Cytokine Production Protocol
Application Flow Cytometry, Functional Assays
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.