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C57Bl/6 splenocytes were stimulated with Cell Stimulation Cocktail (w/o protein transport inhibitors) (500X) (TNB-4970) supplemented with Brefeldin A Solution (TNB-4506) and then stained with APC Anti-Mouse CD3e (20-0031), followed by intracellular staining with PE Anti-Mouse IL-2 (50-7021) (right panel) or PE Rat IgG2b (left panel).
  • C57Bl/6 splenocytes were stimulated with Cell Stimulation Cocktail (w/o protein transport inhibitors) (500X) (TNB-4970) supplemented with Brefeldin A Solution (TNB-4506) and then stained with APC Anti-Mouse CD3e (20-0031), followed by intracellular staining with PE Anti-Mouse IL-2 (50-7021) (right panel) or PE Rat IgG2b (left panel).

Cell Stimulation Cocktail (w/o protein transport inhibitors) (500X)

Cat No. Size Price Quantity
TNB-4970-UL100 100 µL $55.00
TNB-4970-UL400 400 µL (4 x 100 µL) $165.00

Description

Cell Stimulation Cocktail (w/o protein transport inhibitors) (500X) is a ready to use cocktail that can be used for polyclonal cell activation. It is composed of the phorbol ester, PMA (Phorbol 12-Myristate 13-Acetate) and a calcium ionophore (Ionomycin). This Cell Stimulation Cocktail (w/o protein transport inhibitors) (500X) can be used to elicit cytokine production in a variety of cell types allowing for detection in immunoassays.

 

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Cell Stimulation Cocktail (w/o protein transport inhibitors) (500X) is a ready to use cocktail that can be used for polyclonal cell activation. It is composed of the phorbol ester, PMA (Phorbol 12-Myristate 13-Acetate) and a calcium ionophore (Ionomycin). This Cell Stimulation Cocktail (w/o protein transport inhibitors) (500X) can be used to elicit cytokine production in a variety of cell types allowing for detection in immunoassays.

 

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Product Details

Name Cell Stimulation Cocktail (w/o protein transport inhibitors) (500X)
Cat. No. TNB-4970
Application Functional Assays
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.