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Brefeldin A Solution (1000X)
  • Brefeldin A Solution (1000X)

Brefeldin A Solution (1000X)

Cat No. Size Price Quantity
TNB-4506-L001 1 mL $45.00

Description

Brefeldin A (BFA) is a protein transport inhibitor that can be added to cell culture medium during in vitro activation of cells to inhibit the secretion of cytokines elicited by stimulation. Addition of BFA leads to leads to an accumulation of newly synthesized protein in the Golgi complex. The use of Brefeldin A effectively enhances the detection of most mouse and human intracellular cytokines however, other protein transport inhibitors such as Monensin can also be used. It is recommended that investigators evaluate the optimal reagent to use for their specific assay system.

 

Recent Citations: 

Teghanemt A, Pulipati P, Misel-Wuchter K, Day K, Yorek MS, Yi R, Keen HL, Au C, Maretzky T, Gurung P, Littman DR, Issuree PD. CD4 expression in effector T cells depends on DNA demethylation over a developmentally established stimulus-responsive element. Nat Commun. 2022 Mar 18;13(1):1477. doi: 10.1038/s41467-022-28914-4. PMID: 35304452.

» View on NatureCommunications

Lam LKM, Watson AM, Ryman KD, Klimstra WB. Gamma-interferon exerts a critical early restriction on replication and dissemination of yellow fever virus vaccine strain 17D-204. NPJ Vaccines. 2018;3:5. Published 2018 Jan 23. doi:10.1038/s41541-017-0039-z.

View on PubMed

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Brefeldin A (BFA) is a protein transport inhibitor that can be added to cell culture medium during in vitro activation of cells to inhibit the secretion of cytokines elicited by stimulation. Addition of BFA leads to leads to an accumulation of newly synthesized protein in the Golgi complex. The use of Brefeldin A effectively enhances the detection of most mouse and human intracellular cytokines however, other protein transport inhibitors such as Monensin can also be used. It is recommended that investigators evaluate the optimal reagent to use for their specific assay system.

 

Recent Citations: 

Teghanemt A, Pulipati P, Misel-Wuchter K, Day K, Yorek MS, Yi R, Keen HL, Au C, Maretzky T, Gurung P, Littman DR, Issuree PD. CD4 expression in effector T cells depends on DNA demethylation over a developmentally established stimulus-responsive element. Nat Commun. 2022 Mar 18;13(1):1477. doi: 10.1038/s41467-022-28914-4. PMID: 35304452.

» View on NatureCommunications

Lam LKM, Watson AM, Ryman KD, Klimstra WB. Gamma-interferon exerts a critical early restriction on replication and dissemination of yellow fever virus vaccine strain 17D-204. NPJ Vaccines. 2018;3:5. Published 2018 Jan 23. doi:10.1038/s41541-017-0039-z.

View on PubMed

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Product Details

Name Brefeldin A Solution (1000X) ()
Cat. No. TNB-4506
Application Functional Assays
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.