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Human peripheral blood lymphocytes were stained with 0.5 ug Biotin Anti-Human CD45RA (30-0458) (solid line) or 0.5 ug Biotin Mouse IgG2b isotype control (dashed line), followed by Streptavidin PE.
  • Human peripheral blood lymphocytes were stained with 0.5 ug Biotin Anti-Human CD45RA (30-0458) (solid line) or 0.5 ug Biotin Mouse IgG2b isotype control (dashed line), followed by Streptavidin PE.

Biotin Anti-Human CD45RA (HI100)

Cat No. Size Price Quantity
30-0458-U025 25 µg $32.00
30-0458-U100 100 µg $79.00

Description

The HI100 antibody reacts with the human CD45 isoform known as CD45RA, a protein tyrosine phosphatase of ≥ 220 kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns. With their broad cell distribution, CD45 isoforms are critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RB, CD45RO and others.

The HI100 antibody is widely used as a marker for human CD45RA expression on naive and activated T cells, B cells, and monocytes.

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The HI100 antibody reacts with the human CD45 isoform known as CD45RA, a protein tyrosine phosphatase of ≥ 220 kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns. With their broad cell distribution, CD45 isoforms are critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RB, CD45RO and others.

The HI100 antibody is widely used as a marker for human CD45RA expression on naive and activated T cells, B cells, and monocytes.

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Product Details

Name Biotin Anti-Human CD45RA (HI100)
Cat. No. 30-0458
Alternative Names
Gene ID 5788
Clone HI100
Isotype Mouse IgG2b, kappa
Reactivity Human
Cross Reactivity Chimpanzee
Format Biotin
Application Flow Cytometry
Citations*

Kroenke MA, Eto, D, Locci M, Cho M, Davidson T, Haddad EK, and Crotty S. 2012. J. Immunol. 188: 3734-3744. (Flow Cytometry)

Lopez-Verges S, Milush JM, Schwartz BS, Pando MJ, Jarjoura J, York VA, Houchins JP, Miller S, Kang S-M, Norris PJ, Nixon DF, and Lanier LL. 2011. Proc. Natl. Acad. Sci. 108: 14725-14732. (Flow Cytometry)

Imanguli MM, Swaim WD, League SC, Gress RE, Pavletic SZ, and Hakim FT. 2009. Blood. 113: 3620-3630. (Immunohistochemistry - paraffin embedded tissue)

Kim M-H, Suh H-S, Si Q, Terman BE, and Lee SC. 2006. J. Virol. 80: 62-72. (Western Blot)

Weninger W, Carlsen HS, Goodarzi M, Moazed F, Crowley MA, Baekkevold ES, Cavanagh LL, and von Andrian U. 2003. J. Immunol. 170: 4638-4648. (Immunohistochemistry – frozen tissue.

Yamada T, Zhu D, Saxon A, and Zhang K. 2002. J. Biol. Chem. 277(32): 28830-28835. (in vitro blocking)

Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.