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Human peripheral blood lymphocytes were stained with 0.5 ug Biotin Anti-Human CD19 (30-0198) (solid line) or 0.5 ug Biotin Mouse IgG1 isotype control (dashed line), followed by Streptavidin PE.
  • Human peripheral blood lymphocytes were stained with 0.5 ug Biotin Anti-Human CD19 (30-0198) (solid line) or 0.5 ug Biotin Mouse IgG1 isotype control (dashed line), followed by Streptavidin PE.

Biotin Anti-Human CD19 (SJ25C1)

Cat No. Size Price Quantity
30-0198-U025 25 µg $69.00
30-0198-U100 100 µg $170.00

Description

The SJ25C1 antibody reacts with human CD19, a 95 kDa glycoprotein which acts as a co-receptor, along with CD21 (CR2), CD81 (TAPA-1) and CD225 (Leu13), in support of the functional B cell receptor (BCR). This complex provides antigen-specific recognition and subsequent activation of B cells to proliferate and differentiate into antibody-secreting cells (plasma cells) or memory B cells, which are crucial for secondary antigen encounter. Upon activation and tyrosine phosphorylation, the CD19 molecule can provide an anchor for cytoplasmic signaling proteins such as GRB2, SOS or PLCG2. CD19 is a lineage-differentiation marker, as its expression is detectable at the earliest B cell stages, through development, and is finally lost upon transition to mature plasma cells.

The SJ25C1 antibody is widely used as a phenotypic marker for CD19 expression on B cells, as well as on dendritic cell subsets.

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The SJ25C1 antibody reacts with human CD19, a 95 kDa glycoprotein which acts as a co-receptor, along with CD21 (CR2), CD81 (TAPA-1) and CD225 (Leu13), in support of the functional B cell receptor (BCR). This complex provides antigen-specific recognition and subsequent activation of B cells to proliferate and differentiate into antibody-secreting cells (plasma cells) or memory B cells, which are crucial for secondary antigen encounter. Upon activation and tyrosine phosphorylation, the CD19 molecule can provide an anchor for cytoplasmic signaling proteins such as GRB2, SOS or PLCG2. CD19 is a lineage-differentiation marker, as its expression is detectable at the earliest B cell stages, through development, and is finally lost upon transition to mature plasma cells.

The SJ25C1 antibody is widely used as a phenotypic marker for CD19 expression on B cells, as well as on dendritic cell subsets.

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Product Details

Name Biotin Anti-Human CD19 (SJ25C1)
Cat. No. 30-0198
Alternative Names
Gene ID 930
Clone SJ25C1
Isotype Mouse IgG2a, κ
Reactivity Human
Cross Reactivity
Format Biotin
Application Flow Cytometry
Citations*

Piatosa B, Birbach M, Siewiera K, Ussowicz M, Kalwak K, Drabko K, Rekawek A, Tkaczyk K, Kurowski PN. 2013. Cytometry Part B. 84B: 179–186. (Flow Cytometry)

Sekiguchi DR, Smith SB, Sutter JA, Goodman NG, Propert K, Louzoun Y, Rogers W, Luning Prak ET. 2011. Cytometry Part B. 80B: 291–299. (Flow Cytometry)

Dörken B, Möller P, Pezzutto A, Schwartz-Albiez R, Moldenhauer G. B-cell antigens: CD19. In: Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:34-36. (Flow Cytometry)

Karahan GE, Eikmans M, Anholts JDH, Class FHJ and Heidt S. 2014. Clin Exp Immunol. 177(1): 333-340. (Flow Cytometry)

Bansal RR, Mackay CR, Moser B and Ebert M. 2012. Eur J Immunol. 42(1): 110-119. (Flow Cytometry)

Jourdan M, Caraux A, Caron G, Robert N, Fiol G, Rème T, Bolloré K, Vendrell JP, Le Gallou S, Mourcin F, De Vos J, Kassambara A, Duperray C, Hose D, Fest T, Tarte K and Klein B. 2011. J Immunol. 187(8): 3931-3941. (Flow Cytometry)

Goval J-J, Greimers R, Boniver J and de Leval L. 2006. J Histochem Cytochem. 54(1): 75-84. (Immunofluorescence – frozen tissue)

Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.