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C57Bl/6 splenocytes were stained with Biotin Anti-Mouse CD19 (solid line) or Biotin Rat IgG2a isotype control (dashed line), followed by 0.06 ug APC Streptavidin (20-4317).
  • C57Bl/6 splenocytes were stained with Biotin Anti-Mouse CD19 (solid line) or Biotin Rat IgG2a isotype control (dashed line), followed by 0.06 ug APC Streptavidin (20-4317).

APC Streptavidin

Cat No. Size Price Quantity
20-4317-U100 100 µg $59.00
20-4317-U500 500 µg $249.00

Description

APC Streptavidin is a second-step reagent useful when detecting biotinylated antibodies in indirect staining protocols. Streptavidin is a nonglycosylated biotin-binding protein that is bacterially derived. Like avidin, it binds to biotin with high affinity but displays less nonspecific binding.

Recent Publications: 

Jugniot N, Bam R, Paulmurugan R. Expression and purification of a native Thy1-single-chain variable fragment for use in molecular imaging. Sci Rep. 2021 Nov 29;11(1):23026. doi: 10.1038/s41598-021-02445-2. PMID: 34845270; PMCID: PMC8630227.

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Noviski M, Mueller JL, Satterthwaite A, Garrett-Sinha LA, Brombacher F, Zikherman J. IgM and IgD B cell receptors differentially respond to endogenous antigens and control B cell fate. Elife. 2018;7:e35074. Published 2018 Mar 9. doi:10.7554/eLife.35074.

» View on PubMed

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APC Streptavidin is a second-step reagent useful when detecting biotinylated antibodies in indirect staining protocols. Streptavidin is a nonglycosylated biotin-binding protein that is bacterially derived. Like avidin, it binds to biotin with high affinity but displays less nonspecific binding.

Recent Publications: 

Jugniot N, Bam R, Paulmurugan R. Expression and purification of a native Thy1-single-chain variable fragment for use in molecular imaging. Sci Rep. 2021 Nov 29;11(1):23026. doi: 10.1038/s41598-021-02445-2. PMID: 34845270; PMCID: PMC8630227.

» View on PubMed

Noviski M, Mueller JL, Satterthwaite A, Garrett-Sinha LA, Brombacher F, Zikherman J. IgM and IgD B cell receptors differentially respond to endogenous antigens and control B cell fate. Elife. 2018;7:e35074. Published 2018 Mar 9. doi:10.7554/eLife.35074.

» View on PubMed

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Product Details

Name APC Streptavidin
Cat. No. 20-4317
Alternative Names SAv-APC, SA-APC, Streptavidin-Allophycocyanin
Gene ID
Clone
Isotype
Reactivity
Cross Reactivity
Format APC
Application Flow Cytometry
Citations*

Noviski M, Mueller JL, Satterthwaite A, Garrett-Sinha LA, Brombacher F, Zikherman J. IgM and IgD B cell receptors differentially respond to endogenous antigens and control B cell fate. Elife. 2018;7:e35074. Published 2018 Mar 9. doi:10.7554/eLife.35074.

» View on PubMed

Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.