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SJL splenocytes were stained with 1 ug APC-Cy7 Anti-Mouse CD45.1 (25-0453) (solid line) or 1 ug APC-Cy7 Mouse IgG2a isotype control (dashed line).
  • SJL splenocytes were stained with 1 ug APC-Cy7 Anti-Mouse CD45.1 (25-0453) (solid line) or 1 ug APC-Cy7 Mouse IgG2a isotype control (dashed line).

APC-Cyanine7 Anti-Mouse CD45.1 (A20)

Cat No. Size Price Quantity
25-0453-U025 25 µg $63.00
25-0453-U100 100 µg $144.00

Description

The A20 antibody reacts with mouse CD45.1, also known as Ly5.1, which is a strain-specific allelic form of the CD45 Leukocyte Common Antigen (LCA). Functionally, CD45 is a protein tyrosine phosphatase whose broad cell distribution supports a critical role in many leukocyte functions, including regulation of signal transduction and cell activation associated with the T cell and B cell receptors.

The A20 antibody is typically used as a leukocyte marker in Ly5.1 mouse strains: SJL/J, DA, STS/A and RIII. The antibody has been demonstrated to specific for CD45.1 and is not cross-reactive with CD45.2 bearing cells.

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The A20 antibody reacts with mouse CD45.1, also known as Ly5.1, which is a strain-specific allelic form of the CD45 Leukocyte Common Antigen (LCA). Functionally, CD45 is a protein tyrosine phosphatase whose broad cell distribution supports a critical role in many leukocyte functions, including regulation of signal transduction and cell activation associated with the T cell and B cell receptors.

The A20 antibody is typically used as a leukocyte marker in Ly5.1 mouse strains: SJL/J, DA, STS/A and RIII. The antibody has been demonstrated to specific for CD45.1 and is not cross-reactive with CD45.2 bearing cells.

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Product Details

Name APC-Cyanine7 Anti-Mouse CD45.1 (A20)
Cat. No. 25-0453
Alternative Names 25-0453, SJL, Ly5.1
Gene ID 19264
Clone A20
Isotype Mouse IgG2a, κ
Reactivity Mouse
Cross Reactivity
Format APC-Cyanine7
Application Flow Cytometry
Citations*

Shiyang Li, John W. Bostick, Jian Ye, Joseph F. Urban, Jr., Dorina Avram, Liang Zhou. Aryl Hydrocarbon Receptor Signaling Cell Intrinsically Inhibits Intestinal Group 2 Innate Lymphoid Cell Function. Immunity. 2018 Nov 20;49(5):915-928.e5. doi: 10.1016/j.immuni.2018.09.015

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Willinger T and Flavell RA. 2012. Proc. Natl. Acad. Sci. 109: 8670-8675. (Flow Cytometry)

Siggs OM, Li X, Xia Y, and Beutler B. 2012. J. Exp. Med. 209:19-27. (Flow Cytometry)

Wakim LM, Woodward-Davis A and Bevan MJ. 2010. 107: 17872-17879. (Immunohistochemistry – OCT embedded frozen tissue)

Ato M, Nakano H, Kakiuchi T, and Kaye PM. 2004. J. Immunol. 173: 4815-4820. (Immunohistochemistry – frozen tissue)

Shen F-W, Tung J-S and Boyse EA. 1986. Immunogenetics. 24(3): 146-149. (Immunoprecipitation)

Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.