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C57Bl/6 bone marrow cells were stained with violetFluor™ 450 Anti-Mouse CD45R (B220) (75-0452) and 0.25 ug APC-Cyanine7 Anti-Mouse CD117 (25-1172) (right panel) or 0.25 ug  APC-Cyanine7 Rat IgG2b (left panel).
  • C57Bl/6 bone marrow cells were stained with violetFluor™ 450 Anti-Mouse CD45R (B220) (75-0452) and 0.25 ug APC-Cyanine7 Anti-Mouse CD117 (25-1172) (right panel) or 0.25 ug  APC-Cyanine7 Rat IgG2b (left panel).

APC-Cyanine7 Anti-Mouse CD117 (c-Kit) (ACK2)

Cat No. Size Price Quantity
25-1172-U025 25 µg $99.00
25-1172-U100 100µg $189.00

Description

The ACK2 antibody is specific for CD117, also called c-Kit, a 145 kDa cytokine receptor important in the development of hematopoietic stem cells, in oogenesis, and for functional activity of ≥≥immune cells such as NK and mast cells. c-Kit binds to a ligand known as stem cell factor (SCF), or alternatively as mast cell growth factor. Ligand binding promotes the activation (dimerization) and subsequent tyrosine kinase activity of ≥≥the c-Kit receptor and triggers key survival, expansion and maturation signals during hematopoietic progenitor cell development. Conversely, shedding of extracellular domain of c-Kit receptor is reported to induce inactivation or apoptosis within these cells. The survival signaling activity of ≥≥c-Kit confers a proto-oncogenic attribute to the receptor, as overexpression or mutations in this protein are associated with tumor development.

The ACK2 antibody is widely utilized as a marker to identify hematopoietic progenitors, and to neutralize receptor-ligand binding in vitro and in vivo. Please choose the appropriate format for each application..

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The ACK2 antibody is specific for CD117, also called c-Kit, a 145 kDa cytokine receptor important in the development of hematopoietic stem cells, in oogenesis, and for functional activity of ≥≥immune cells such as NK and mast cells. c-Kit binds to a ligand known as stem cell factor (SCF), or alternatively as mast cell growth factor. Ligand binding promotes the activation (dimerization) and subsequent tyrosine kinase activity of ≥≥the c-Kit receptor and triggers key survival, expansion and maturation signals during hematopoietic progenitor cell development. Conversely, shedding of extracellular domain of c-Kit receptor is reported to induce inactivation or apoptosis within these cells. The survival signaling activity of ≥≥c-Kit confers a proto-oncogenic attribute to the receptor, as overexpression or mutations in this protein are associated with tumor development.

The ACK2 antibody is widely utilized as a marker to identify hematopoietic progenitors, and to neutralize receptor-ligand binding in vitro and in vivo. Please choose the appropriate format for each application..

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Product Details

Name APC-Cyanine7 Anti-Mouse CD117 (c-Kit) (ACK2)
Cat. No. 25-1172
Alternative Names Steel Factor Receptor, SCFR, cKit
Gene ID 16590
Clone ACK2
Isotype Rat IgG2b, κ
Reactivity Mouse
Cross Reactivity Rat
Format APC-Cyanine7
Application Flow Cytometry
Citations*

Tang X, Tian L, Esteso G, Choi S-C, Barrow AD, Colonna M, Borrego F, and Coligan JE. 2012. J. Immunol. 188: 548-558. (Flow cytometry)

Launay J-M, Herve P, Callebert J, Mallat Z, Collet C, Doly S, Belmer A, Diaz SL, Cote F, Humbert M, and Maroteaux L. 2012. Blood. 119: 1772-1780. (Immunohistochemistry - formaldehyde fixed tissue)

Mark-Kappeler CJ, Sen N, Lukefahr A, McKee L, Sipes IG, Konhilas J, and Hoyer PB. 2011. Biol. Reprod. 85: 755-762. (in vitro blocking, Western blot – Fischer 344 Rat)

Kim M-H, Granick JL, Wkok C, Walker NJ, Borjesson DL, Curry F-RE, Miller LS, and Simon SI. 2011. Blood. 117:3343-3352. (in vivo depletion)

Fiorina P, Jurewicz M, Vergani A, Petrelli A, Carvello M, D’Addio F et al. 2011. J. Immunol. 186:121-131. (in vivo blocking)

Stanich JE, Gibbons SJ, Eisenman ST, Bardsley MR, Rock JR, Harfe BD, Ordog T, and Farrugia G. 2011. 301: G1044-G1051. (Immunocytochemistry - acetone fixed cells)

Carlsson IB, Laitinen MPE, Scott JE, Louhio H, Velentzis L, Tuuri T, Aaltonen J, Ritvos O, Winston RML, and Hovatta O. 2006. Reproduction. 131: 641-649. (Immunohistochemistry – paraffin embedded tissue, in vivo blocking)

Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.