APC Anti-Mouse F4/80 Antigen (BM8.1)
| Cat No. | Size | Price | Quantity | |
|---|---|---|---|---|
| 20-4801-U025 | 25 µg | $39.00 | ||
| 20-4801-U100 | 100 µg | $115.00 |
Technical Data Sheet
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The BM8.1 antibody is specific for mouse F4/80 antigen, a 125 kDa transmembrane protein widely expressed by members of the mononuclear phagocyte system and considered to be a key marker for mature macrophage cells. F4/80 is differentially expressed during myeloid cell development, and may be regulated by certain cytokines within the tissue microenvironment. Other cell types shown to express this antigen include Langerhans cells, Kupffer cells and dendritic cell subsets. BM8.1 is widely used together with antibodies to CD115 (c-fms), CD11b and CD11c to identify myeloid / macrophage cells by flow cytometry.
Recent Publications:
Taniguchi H, Caeser R, Chavan SS, Zhan YA, Chow A, Manoj P, Uddin F, Kitai H, Qu R, Hayatt O, Shah NS, Quintanal Villalonga Á, Allaj V, Nguyen EM, Chan J, Michel AO, Mukae H, de Stanchina E, Rudin CM, Sen T. WEE1 inhibition enhances the antitumor immune response to PD-L1 blockade by the concomitant activation of STING and STAT1 pathways in SCLC. Cell Rep. 2022 May 17;39(7):110814. doi: 10.1016/j.celrep.2022.110814. PMID: 35584676.
Tan X, Banerjee P, Shi L, Xiao GY, Rodriguez BL, Grzeskowiak CL, Liu X, Yu J, Gibbons DL, Russell WK, Creighton CJ, Kurie JM. p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi. Sci Adv. 2021 Jun 18;7(25):eabf4885. doi: 10.1126/sciadv.abf4885. PMID: 34144984.
Markman, J.L., Porritt, R.A., Wakita, D. et al. Loss of testosterone impairs anti-tumor neutrophil function. Nat Commun 11, 1613 (2020). https://doi.org/10.1038/s41467-020-15397-4.
» View on Nature Communications
Udden SN, Kwak YT, Godfrey V, et al. NLRP12 suppresses hepatocellular carcinoma via downregulation of cJun N-terminal kinase activation in the hepatocyte. Elife. 2019;8:e40396. Published 2019 Apr 16. doi:10.7554/eLife.40396.
Wu J and Waxman DJ. 2015. OncoImmunol. DOI:10.1080/2162402X.2015.1005521. (Flow Cytometry)
The BM8.1 antibody is specific for mouse F4/80 antigen, a 125 kDa transmembrane protein widely expressed by members of the mononuclear phagocyte system and considered to be a key marker for mature macrophage cells. F4/80 is differentially expressed during myeloid cell development, and may be regulated by certain cytokines within the tissue microenvironment. Other cell types shown to express this antigen include Langerhans cells, Kupffer cells and dendritic cell subsets. BM8.1 is widely used together with antibodies to CD115 (c-fms), CD11b and CD11c to identify myeloid / macrophage cells by flow cytometry.
Recent Publications:
Taniguchi H, Caeser R, Chavan SS, Zhan YA, Chow A, Manoj P, Uddin F, Kitai H, Qu R, Hayatt O, Shah NS, Quintanal Villalonga Á, Allaj V, Nguyen EM, Chan J, Michel AO, Mukae H, de Stanchina E, Rudin CM, Sen T. WEE1 inhibition enhances the antitumor immune response to PD-L1 blockade by the concomitant activation of STING and STAT1 pathways in SCLC. Cell Rep. 2022 May 17;39(7):110814. doi: 10.1016/j.celrep.2022.110814. PMID: 35584676.
Tan X, Banerjee P, Shi L, Xiao GY, Rodriguez BL, Grzeskowiak CL, Liu X, Yu J, Gibbons DL, Russell WK, Creighton CJ, Kurie JM. p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi. Sci Adv. 2021 Jun 18;7(25):eabf4885. doi: 10.1126/sciadv.abf4885. PMID: 34144984.
Markman, J.L., Porritt, R.A., Wakita, D. et al. Loss of testosterone impairs anti-tumor neutrophil function. Nat Commun 11, 1613 (2020). https://doi.org/10.1038/s41467-020-15397-4.
» View on Nature Communications
Udden SN, Kwak YT, Godfrey V, et al. NLRP12 suppresses hepatocellular carcinoma via downregulation of cJun N-terminal kinase activation in the hepatocyte. Elife. 2019;8:e40396. Published 2019 Apr 16. doi:10.7554/eLife.40396.
Wu J and Waxman DJ. 2015. OncoImmunol. DOI:10.1080/2162402X.2015.1005521. (Flow Cytometry)
Product Details
| Name | APC Anti-Mouse F4/80 Antigen (BM8.1) |
|---|---|
| Cat. No. | 20-4801 |
| Alternative Names | EMR1, Ly71 |
| Gene ID | 13733 |
| Clone | BM8.1 |
| Isotype | Rat IgG2b, κ |
| Reactivity | Mouse |
| Cross Reactivity | |
| Format | APC |
| Application | Flow Cytometry |
| Citations* |
Markman, J.L., Porritt, R.A., Wakita, D. et al. Loss of testosterone impairs anti-tumor neutrophil function. Nat Commun 11, 1613 (2020). https://doi.org/10.1038/s41467-020-15397-4. » View on Nature Communications Udden SN, Kwak YT, Godfrey V, et al. NLRP12 suppresses hepatocellular carcinoma via downregulation of cJun N-terminal kinase activation in the hepatocyte. Elife. 2019;8:e40396. Published 2019 Apr 16. doi:10.7554/eLife.40396. Ioannou M, Alissafi T, Boon L, Boumpas D, and Verginis P. 2013. J. Immunol. 190: 2631-2640. (Flow Cytometry) Papadopoulos G, Weinberg EO, Massari P, Gibson FC, Wetzler LM, Morgan EF, and Genco CA. 2013. J. Immunol. 190: 1148-1157. Chen Q and Snapper CM. 2013. J. Immunol. 190: 1048-1055. Rankin AL, Mumm JB, Murphy E, Turner S, Yu N, McClanahan TK, Bourne PA, Pierce RH, Kastelein R and Pflanz S. 2010. J. Immunol. 184(3): 1526-1535. (Immunohistochemistry - paraffin-embedded tissue) Geutskens SB, Otonkoski T, Pulkkinen MA, Drexhage HA and Leenen PJ. 2005. J. Leukoc. Biol. 78(4): 845-52 (Immunohistochemistry - frozen tissue) |
FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.