APC Anti-Mouse CD45.2 (104)
| Cat No. | Size | Price | Quantity | |
|---|---|---|---|---|
| 20-0454-U025 | 25 µg | $30.00 | ||
| 20-0454-U100 | 100 µg | $105.00 |
Technical Data Sheet
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The 104 antibody reacts with mouse CD45.2, also known as Ly5.2, which is a strain-specific allelic form of the CD45 Leukocyte Common Antigen (LCA). Functionally, CD45 is a protein tyrosine phosphatase whose broad cell distribution supports a critical role in many leukocyte functions, including regulation of signal transduction and cell activation associated with the T cell and B cell receptors.
The 104 antibody is typically used as a leukocyte marker in Ly5.2 mouse strains C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A and AKR. The antibody has been demonstrated to be specific for CD45.2 and is not cross-reactive with CD45.1-bearing cells.
Recent Publications:
Rahim T, Levinson MA, Carufe KEW, Burak M, Meas R, Maher S, Bothwell ALM, Gades N, Sweasy JB. The hematopoietic compartment is sufficient for lupus development resulting from the POLB-Y265C mutation. PLoS One. 2022 Apr 29;17(4):e0267913. doi: 10.1371/journal.pone.0267913. PMID: 35486639; PMCID: PMC9053796.
Nishina T, Deguchi Y, Ohshima D, et a. Interleukin-11-expressing fibroblasts have a unique gene signature correlated with poor prognosis of colorectal cancer. Nat Commun. 2021 Apr 16;12(1):2281. doi: 10.1038/s41467-021-22450-3. PMID: 33863879.
» View on Nature Communications
Mishra S, Liao W, Liu Y, Yang M, Ma C, Wu H, Zhao M, Zhang X, Qiu Y, Lu Q, Zhang N. TGF-β and Eomes control the homeostasis of CD8+ regulatory T cells. J Exp Med. 2021 Jan 4;218(1):e20200030. doi: 10.1084/jem.20200030. PMID: 32991667; PMCID: PMC7527976.
Forte D, García-Fernández M, Sánchez-Aguilera A, Stavropoulou V, Fielding C, Martín-Pérez D, López JA, Costa ASH, Tronci L, Nikitopoulou E, Barber M, Gallipoli P, Marando L, Fernández de Castillejo CL, Tzankov A, Dietmann S, Cavo M, Catani L, Curti A, Vázquez J, Frezza C, Huntly BJ, Schwaller J, Méndez-Ferrer S. Bone Marrow Mesenchymal Stem Cells Support Acute Myeloid Leukemia Bioenergetics and Enhance Antioxidant Defense and Escape from Chemotherapy. Cell Metab. 2020 Sep 16:S1550-4131(20)30479-4. doi: 10.1016/j.cmet.2020.09.001. PMID: 32966766.
The 104 antibody reacts with mouse CD45.2, also known as Ly5.2, which is a strain-specific allelic form of the CD45 Leukocyte Common Antigen (LCA). Functionally, CD45 is a protein tyrosine phosphatase whose broad cell distribution supports a critical role in many leukocyte functions, including regulation of signal transduction and cell activation associated with the T cell and B cell receptors.
The 104 antibody is typically used as a leukocyte marker in Ly5.2 mouse strains C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A and AKR. The antibody has been demonstrated to be specific for CD45.2 and is not cross-reactive with CD45.1-bearing cells.
Recent Publications:
Rahim T, Levinson MA, Carufe KEW, Burak M, Meas R, Maher S, Bothwell ALM, Gades N, Sweasy JB. The hematopoietic compartment is sufficient for lupus development resulting from the POLB-Y265C mutation. PLoS One. 2022 Apr 29;17(4):e0267913. doi: 10.1371/journal.pone.0267913. PMID: 35486639; PMCID: PMC9053796.
Nishina T, Deguchi Y, Ohshima D, et a. Interleukin-11-expressing fibroblasts have a unique gene signature correlated with poor prognosis of colorectal cancer. Nat Commun. 2021 Apr 16;12(1):2281. doi: 10.1038/s41467-021-22450-3. PMID: 33863879.
» View on Nature Communications
Mishra S, Liao W, Liu Y, Yang M, Ma C, Wu H, Zhao M, Zhang X, Qiu Y, Lu Q, Zhang N. TGF-β and Eomes control the homeostasis of CD8+ regulatory T cells. J Exp Med. 2021 Jan 4;218(1):e20200030. doi: 10.1084/jem.20200030. PMID: 32991667; PMCID: PMC7527976.
Forte D, García-Fernández M, Sánchez-Aguilera A, Stavropoulou V, Fielding C, Martín-Pérez D, López JA, Costa ASH, Tronci L, Nikitopoulou E, Barber M, Gallipoli P, Marando L, Fernández de Castillejo CL, Tzankov A, Dietmann S, Cavo M, Catani L, Curti A, Vázquez J, Frezza C, Huntly BJ, Schwaller J, Méndez-Ferrer S. Bone Marrow Mesenchymal Stem Cells Support Acute Myeloid Leukemia Bioenergetics and Enhance Antioxidant Defense and Escape from Chemotherapy. Cell Metab. 2020 Sep 16:S1550-4131(20)30479-4. doi: 10.1016/j.cmet.2020.09.001. PMID: 32966766.
Product Details
| Name | APC Anti-Mouse CD45.2 (104) |
|---|---|
| Cat. No. | 20-0454 |
| Alternative Names | Ly5.2 |
| Gene ID | 19264 |
| Clone | 104 |
| Isotype | Mouse IgG2a, κ |
| Reactivity | Mouse |
| Cross Reactivity | |
| Format | APC |
| Application | Flow Cytometry |
| Citations* |
Dolina JS, Lee J, Griswold RQ, Labarta-Bajo L, Kannan S, Greenbaum JA, Bahia El Idrissi N, Pont MJ, Croft M, Schoenberger SP. TLR9 Sensing of Self-DNA Controls Cell-Mediated Immunity to Listeria Infection via Rapid Conversion of Conventional CD4+ T Cells to Treg. Cell Rep. 2020 Apr 7;31(1):107249. doi: 10.1016/j.celrep.2020.01.040. Willinger T and Flavell, RA. 2012. Proc. Natl. Acad. Sci. 109: 8670 - 8675. (Flow Cytometry) Hale JS, Nelson LT, Simmons KB, and Fink PJ. 2011. J. Immunol. 186: 799 - 806. (Flow Cytometry) Orr MT, Beilke JN, Proekt I, and Lanier LL. 2010. Proc. Natl. Acad. Sci. 107: 15844 - 15849. (Flow Cytometry) Banerjee K, Biswas PS, Kumaraguru U, Schoenberger SP, and Rouse BT. 2004. 173: 7575-7583. (Immunofluorescence microscopy – frozen tissue) Favre CJ, Mancuso M, Maas K, McLean JW, Baluk P, and Mcdonald DM. 2003. Am. J. Physiol. Heart Circ. Physiol. 285:H1917-H1938. (Immunocytochemistry) Shen F-W, Tung J-S, and Boyse EA. 1986. Immunogenetics. 24(3): 146-149. (Immunoprecipitation). |
FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.