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C57Bl/6 (left panel) or SJL (right panel) splenocytes were stained with 0.5 ug APC Anti-Mouse CD45.1 (20-0453) (solid line) or 0.5 ug APC Mouse IgG2a isotype control (dashed line).
  • C57Bl/6 (left panel) or SJL (right panel) splenocytes were stained with 0.5 ug APC Anti-Mouse CD45.1 (20-0453) (solid line) or 0.5 ug APC Mouse IgG2a isotype control (dashed line).
  • APC Anti-Mouse CD45.1 (A20)

APC Anti-Mouse CD45.1 (A20)

Cat No. Size Price Quantity
20-0453-U025 25 µg $51.00
20-0453-U100 100 µg $135.00

Description

The A20 antibody reacts with mouse CD45.1, also known as Ly5.1, which is a strain-specific allelic form of the CD45 Leukocyte Common Antigen (LCA). Functionally, CD45 is a protein tyrosine phosphatase whose broad cell distribution supports a critical role in many leukocyte functions, including regulation of signal transduction and cell activation associated with the T cell and B cell receptors.

The A20 antibody is typically used as a leukocyte marker in Ly5.1 mouse strains: SJL/J, DA, STS/A and RIII. The antibody has been demonstrated to specific for CD45.1 and is not cross-reactive with CD45.2 bearing cells.

Recent Publications:
Miro-Mur F, Perez-de Puig I, Ferrer-Ferrer M, Urra X, Justicia C, Chamorro A and Planas AM. 2015. Brain Behav Immun. doi: 10.1016/j.bbi.2015.08.010. (Flow Cytometry)

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The A20 antibody reacts with mouse CD45.1, also known as Ly5.1, which is a strain-specific allelic form of the CD45 Leukocyte Common Antigen (LCA). Functionally, CD45 is a protein tyrosine phosphatase whose broad cell distribution supports a critical role in many leukocyte functions, including regulation of signal transduction and cell activation associated with the T cell and B cell receptors.

The A20 antibody is typically used as a leukocyte marker in Ly5.1 mouse strains: SJL/J, DA, STS/A and RIII. The antibody has been demonstrated to specific for CD45.1 and is not cross-reactive with CD45.2 bearing cells.

Recent Publications:
Miro-Mur F, Perez-de Puig I, Ferrer-Ferrer M, Urra X, Justicia C, Chamorro A and Planas AM. 2015. Brain Behav Immun. doi: 10.1016/j.bbi.2015.08.010. (Flow Cytometry)

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Product Details

Name APC Anti-Mouse CD45.1 (A20)
Cat. No. 20-0453
Alternative Names SJL, Ly5.1
Gene ID 19264
Clone A20
Isotype Mouse IgG2a, κ
Reactivity Mouse
Cross Reactivity
Format APC
Application Flow Cytometry
Citations*

Willinger T and Flavell RA. 2012. Proc. Natl. Acad. Sci. 109: 8670-8675. (Flow Cytometry)

Siggs OM, Li X, Xia Y, and Beutler B. 2012. J. Exp. Med. 209:19-27. (Flow Cytometry)

Wakim LM, Woodward-Davis A and Bevan MJ. 2010. 107: 17872-17879. (Immunohistochemistry – OCT embedded frozen tissue)

Ato M, Nakano H, Kakiuchi T, and Kaye PM. 2004. J. Immunol. 173: 4815-4820. (Immunohistochemistry – frozen tissue)

Shen F-W, Tung J-S and Boyse EA. 1986. Immunogenetics. 24(3): 146-149. (Immunoprecipitation)

Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.