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C57Bl/6 splenocytes were stimulated with anti-IgM and anti-CD40 for 4 days. Cells were then stained with 0.25 ug APC Anti-Mouse CD252 (20-5905) (solid line) or 0.25 ug APC Rat IgG2b isotype control (dashed line).
  • C57Bl/6 splenocytes were stimulated with anti-IgM and anti-CD40 for 4 days. Cells were then stained with 0.25 ug APC Anti-Mouse CD252 (20-5905) (solid line) or 0.25 ug APC Rat IgG2b isotype control (dashed line).

APC Anti-Mouse CD252 (OX40 Ligand) (RM134L)

Cat No. Size Price Quantity
20-5905-U025 25 µg $51.00
20-5905-U100 100 µg $136.00

Description

The RM134L antibody recognizes CD252, also known as OX-40 Ligand or CD134 Ligand, a member of the TNF-α superfamily that is present on the surface of antigen presenting cells and activated B lymphocytes. The OX-40 Ligand interacts with OX-40 (CD134) which is expressed primarily on activated T cells. This costimulatory interaction leads to increased proliferation and IL-2 production responses of activated T cells, and at the same time enhances proliferation and immunoglobulin secretion by activated B cells.

The RM134L antibody is useful for flow cytometric detection of CD252 on stimulated mouse splenocytes. It has also been reported to block the costimulatory activity of ≥≥OX-40 Ligand. Please choose the appropriate format for each application.

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The RM134L antibody recognizes CD252, also known as OX-40 Ligand or CD134 Ligand, a member of the TNF-α superfamily that is present on the surface of antigen presenting cells and activated B lymphocytes. The OX-40 Ligand interacts with OX-40 (CD134) which is expressed primarily on activated T cells. This costimulatory interaction leads to increased proliferation and IL-2 production responses of activated T cells, and at the same time enhances proliferation and immunoglobulin secretion by activated B cells.

The RM134L antibody is useful for flow cytometric detection of CD252 on stimulated mouse splenocytes. It has also been reported to block the costimulatory activity of ≥≥OX-40 Ligand. Please choose the appropriate format for each application.

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Product Details

Name APC Anti-Mouse CD252 (OX40 Ligand) (RM134L)
Cat. No. 20-5905
Alternative Names CD134L, CD134 Ligand, OX-40L, OX-40 Ligand, TNF-αSF4, Tumor necrosis factor ligand superfamily member 4, gp34
Gene ID 22164
Clone RM134L
Isotype Rat IgG2b, κ
Reactivity Mouse
Cross Reactivity
Format APC
Application Flow Cytometry
Citations*

Akiba H, Oshima H, Takeda K, Atsuta M, Nakano H, Nakajima A, Nohara C, Yagita H and Okumura K. 1999 J Immunol. 162(12): 7058-7066. (Flow cytometry, in vitro blocking)

Stuber E, Neurath M, Calderhead D, Fell HP and Strober W. 1995 Immunity. 2(5): 507-521.

Sibilano R, Frossi B, Suzuki R, D'Incà F, Gri G, Piconese S, Colombo MP, Rivera J and Pucillo CE. 2012 Journal of Allergy Clin Immunol. 130(3): 751–760. (Blocking)

van der Merwe M, Abdelsamed HA, Seth A, Ong T, Vogel P and Pillai AB. 2013 J Immunol. 191(11): 5764-5776 (Flow cytometry)

Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.