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Untransfected (left) or GARP transfected (right) cells were stained with 5 uL (0.5 ug) APC Anti-Human GARP (20-9882) (solid line) or 0.5 ug APC Mouse IgG1 isotype control (dashed line).
  • Untransfected (left) or GARP transfected (right) cells were stained with 5 uL (0.5 ug) APC Anti-Human GARP (20-9882) (solid line) or 0.5 ug APC Mouse IgG1 isotype control (dashed line).

APC Anti-Human GARP (GARP5)

Cat No. Size Price Quantity
20-9882-T025 25 tests $104.00
20-9882-T100 100 tests $231.00

Description

The GARP5 antibody reacts with human GARP, also known as LRRC32 or Garpin, an 80 kDa transmembrane protein which acts as a receptor for the latent form of TGF-beta 1 (pro-TGF-beta), preventing its secretion. Specifically, GARP is reported to associate with the pro-domain of TGF-beta 1, known as latency-associated peptide (LAP), which must be cleaved to release the biologically active cytokine. The putative role of GARP in sequestering the TGF-beta is important for regulating its activity, as TGF-beta signaling is involved in development and function of Th17, Treg and many other immune cell types. GARP expression has been shown on platelets and at high levels on Foxp3+ Treg cells, where it is proposed to be a phenotypic identifier for activated human Treg cells.

The GARP5 antibody may be used for analysis of human GARP by flow cytometry, immunoprecipitation and Western blotting. Please choose the appropriate format for each application.

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The GARP5 antibody reacts with human GARP, also known as LRRC32 or Garpin, an 80 kDa transmembrane protein which acts as a receptor for the latent form of TGF-beta 1 (pro-TGF-beta), preventing its secretion. Specifically, GARP is reported to associate with the pro-domain of TGF-beta 1, known as latency-associated peptide (LAP), which must be cleaved to release the biologically active cytokine. The putative role of GARP in sequestering the TGF-beta is important for regulating its activity, as TGF-beta signaling is involved in development and function of Th17, Treg and many other immune cell types. GARP expression has been shown on platelets and at high levels on Foxp3+ Treg cells, where it is proposed to be a phenotypic identifier for activated human Treg cells.

The GARP5 antibody may be used for analysis of human GARP by flow cytometry, immunoprecipitation and Western blotting. Please choose the appropriate format for each application.

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Product Details

Name APC Anti-Human GARP (GARP5)
Cat. No. 20-9882
Alternative Names LRRC32, Garpin
Gene ID 2615
Clone GARP5
Isotype Mouse IgG1
Reactivity Human
Cross Reactivity
Format APC
Application Flow Cytometry
Citations*

Wang I, Zhu J, Dong X, Shi M, Lu C, and Springer TA. 2012. Mol. Biol. Cell. 23: 1129 – 1139. (Flow cytometry, Immunoprecipitation, Western blot).

Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.