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Human PBMC were stimulated for 3 days with PHA and stained with 5 uL (2 ug) APC Anti-Human CD274 (20-5984) (solid line) or 2 ug APC Mouse IgG2b isotype control (dashed line).
  • Human PBMC were stimulated for 3 days with PHA and stained with 5 uL (2 ug) APC Anti-Human CD274 (20-5984) (solid line) or 2 ug APC Mouse IgG2b isotype control (dashed line).

APC Anti-Human CD274 (PD-L1, B7-H1) (29E.2A3)

Cat No. Size Price Quantity
20-5984-T025 25 tests $120.00
20-5984-T100 100 tests $260.00

Description

The 29E.2A3 antibody reacts with human CD274 which is also known as PD-L1 or B7-H1. CD274 is a type 1 transmembrane glycoprotein and a member of the Ig superfamily. PD-L1 is a ligand for CD279 (PD-1) and plays an important role in regulating immune cell responses. CD274 is expressed on activated monocytes, macrophages, dendritic cells, and T cells.

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The 29E.2A3 antibody reacts with human CD274 which is also known as PD-L1 or B7-H1. CD274 is a type 1 transmembrane glycoprotein and a member of the Ig superfamily. PD-L1 is a ligand for CD279 (PD-1) and plays an important role in regulating immune cell responses. CD274 is expressed on activated monocytes, macrophages, dendritic cells, and T cells.

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Product Details

Name APC Anti-Human CD274 (PD-L1, B7-H1) (29E.2A3)
Cat. No. 20-5984
Alternative Names PD-L1, B7-H1
Gene ID 29126
Clone 29E.2A3
Isotype Mouse IgG2b, kappa
Reactivity Human
Cross Reactivity
Format APC
Application Flow Cytometry
Citations*

Freeman GJ, Long AJ, Iwai Y, et al. 2000. J Exp Med. 192:1027-1034.

Latchman Y, Wood CR, Chernova T, et al. 2001. Nat Immunol. 2(3):261-268.

Bennett F, Luxenberg D, Ling V, et al. 2003. J Immunol. 170:711-718.

Brown JA, Dorfman DM, Ma FR, et al. 2003. J Immunol. 170:1257-1266.

Carter L, Fouser LA, Jussif J, et al. 2002. Eur J Immunol. 32:634-643.
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.