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Human PBMCs were stimulated with PMA + Ionomycin for 5 hours and then stained stained with 5 uL (0.25 ug) APC Anti-Human CD154 (20-1547) (solid line) or 0.25 ug APC Mouse IgG2a isotype control (dashed line).
  • Human PBMCs were stimulated with PMA + Ionomycin for 5 hours and then stained stained with 5 uL (0.25 ug) APC Anti-Human CD154 (20-1547) (solid line) or 0.25 ug APC Mouse IgG2a isotype control (dashed line).

APC Anti-Human CD154 (CD40L) (5C8)

Cat No. Size Price Quantity
20-1547-T025 25 tests $119.00
20-1547-T100 100 tests $290.00

Description

The 5C8 antibody reacts with human CD154 which is also known as CD40L (CD40 ligand). CD154 is a 39kDa member of the TNF-α superfamily and is predominantly expressed on activated T cells but is also expressed on NK cells, mast cells, basophils, and eosinophils. CD154 binds to CD40, a molecule that is expressed on antigen presenting cells including B cells, monocytes/macrophages, and dendritic cells. The interaction of CD154 and CD40 regulates the activation of antigen presenting cells and is critical for B cell costimulation. The 5C8 antibody has been reported to block CD40L-CD40 interactions.

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The 5C8 antibody reacts with human CD154 which is also known as CD40L (CD40 ligand). CD154 is a 39kDa member of the TNF-α superfamily and is predominantly expressed on activated T cells but is also expressed on NK cells, mast cells, basophils, and eosinophils. CD154 binds to CD40, a molecule that is expressed on antigen presenting cells including B cells, monocytes/macrophages, and dendritic cells. The interaction of CD154 and CD40 regulates the activation of antigen presenting cells and is critical for B cell costimulation. The 5C8 antibody has been reported to block CD40L-CD40 interactions.

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Product Details

Name APC Anti-Human CD154 (CD40L) (5C8)
Cat. No. 20-1547
Alternative Names CD40L, gp39, T-BAM, TRAP, Ly-62
Gene ID 959
Clone 5C8
Isotype Mouse IgG2a, κ
Reactivity Human
Cross Reactivity rhesus monkey, cynomolgus monkey
Format APC
Application Flow Cytometry
Citations* Lederman S, Yellin MJ, Krichevsky A, Belko J, Lee JJ, Chess L. 1992. J Exp Med. 175(4):1091-1101.Lederman S, Yellin MJ, Inghirami G, Lee JJ, Knowles DM, Chess L. 1992. J Immunol. 149(12):3817-3826.Nishioka Y, Lipsky PE. 1994. J Immunol. 153(3):1027-1036. Nadazdin, O., et al. 2011. J Immunol. 187(9): 4589-4597.
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.