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7-AAD
  • 7-AAD

7-AAD

Cat No. Size Price Quantity
13-6993-T200 200 tests $17.00
13-6993-T500 500 tests $35.00

Description

7-AAD (7-Aminoactinomycin D) is a nucleic acid dye that can be used to exclude nonviable cells from the analysis of flow cytometry data.

Recent Publications:

Ornell KJ, Chiu B, Coburn JM. Development of a Dinutuximab Delivery System Using Silk Foams for GD2 Targeted Neuroblastoma Cell Death. J Biomed Mater Res A. 2020 Nov 30. doi: 10.1002/jbm.a.37131. PMID: 33252182.

» View on PubMed

Yonebayashi S, Tajiri K, Murakoshi N, Xu D, et al. MAIR-II deficiency ameliorates cardiac remodelling post-myocardial infarction by suppressing TLR9-mediated macrophage activation. J Cell Mol Med. 2020 Nov 2. doi: 10.1111/jcmm.16070. PMID: 33140535.   

» View on PubMed

Matsuo T, Tashiro H, Sumiyoshi R, et al. Functional expression cloning of molecules inducing CD34 expression in bone marrow-derived stromal myofibroblasts. Biochem Biophys Res Commun. 2020 Oct 13:S0006-291X(20)31909-4. doi: 10.1016/j.bbrc.2020.10.006. PMID: 33066959. 

» View on PubMed

Takahashi D, Hoshina N, Kabumoto Y, et al. Microbiota-derived butyrate limits the autoimmune response by promoting the differentiation of follicular regulatory T cells. EBioMedicine. 2020;58:102913. doi:10.1016/j.ebiom.2020.102913.

» View on ScienceDirect

Domínguez-Romero AN, Martínez-Cortés F, Munguía ME, et al Generation of multiepitope cancer vaccines based on large combinatorial libraries of survivin-derived mutant epitopes [published online ahead of print, 2020 Jul 3]. Immunology. 2020;10.1111/imm.13233. doi:10.1111/imm.13233.

 View on PubMed

Jonathan J Pinney, Fátima Rivera-Escalera, Charles C Chu, et al. Macrophage hypophagia as a mechanism of innate immune exhaustion in mAb-induced cell clearance. Blood.2020005571. doi: https://doi.org/10.1182/blood.2020005571.

» View on PubMed

Kamachi F, Isshiki T, Harada N, Akiba H and Miyake S. 2015. Biochem Biophys Res Commun. doi: 10.1016/j.bbrc.2015.06.005. (Flow Cytometry)

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7-AAD (7-Aminoactinomycin D) is a nucleic acid dye that can be used to exclude nonviable cells from the analysis of flow cytometry data.

Recent Publications:

Ornell KJ, Chiu B, Coburn JM. Development of a Dinutuximab Delivery System Using Silk Foams for GD2 Targeted Neuroblastoma Cell Death. J Biomed Mater Res A. 2020 Nov 30. doi: 10.1002/jbm.a.37131. PMID: 33252182.

» View on PubMed

Yonebayashi S, Tajiri K, Murakoshi N, Xu D, et al. MAIR-II deficiency ameliorates cardiac remodelling post-myocardial infarction by suppressing TLR9-mediated macrophage activation. J Cell Mol Med. 2020 Nov 2. doi: 10.1111/jcmm.16070. PMID: 33140535.   

» View on PubMed

Matsuo T, Tashiro H, Sumiyoshi R, et al. Functional expression cloning of molecules inducing CD34 expression in bone marrow-derived stromal myofibroblasts. Biochem Biophys Res Commun. 2020 Oct 13:S0006-291X(20)31909-4. doi: 10.1016/j.bbrc.2020.10.006. PMID: 33066959. 

» View on PubMed

Takahashi D, Hoshina N, Kabumoto Y, et al. Microbiota-derived butyrate limits the autoimmune response by promoting the differentiation of follicular regulatory T cells. EBioMedicine. 2020;58:102913. doi:10.1016/j.ebiom.2020.102913.

» View on ScienceDirect

Domínguez-Romero AN, Martínez-Cortés F, Munguía ME, et al Generation of multiepitope cancer vaccines based on large combinatorial libraries of survivin-derived mutant epitopes [published online ahead of print, 2020 Jul 3]. Immunology. 2020;10.1111/imm.13233. doi:10.1111/imm.13233.

 View on PubMed

Jonathan J Pinney, Fátima Rivera-Escalera, Charles C Chu, et al. Macrophage hypophagia as a mechanism of innate immune exhaustion in mAb-induced cell clearance. Blood.2020005571. doi: https://doi.org/10.1182/blood.2020005571.

» View on PubMed

Kamachi F, Isshiki T, Harada N, Akiba H and Miyake S. 2015. Biochem Biophys Res Commun. doi: 10.1016/j.bbrc.2015.06.005. (Flow Cytometry)

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Product Details

Name 7-AAD
Cat. No. 13-6993
Protocol Propidium Iodide or 7-AAD Viability Staining Protocol
Excitation Laser Blue (488 nm), Green (532 nm), Yellow-Green (561 nm)
Emission (nm) 647
Formulation 10 mM NaH2PO4, 150 mM Na10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, pH 7.2Cl, 0.09% NaN3, pH 7.2
Application Flow Cytometry
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.