7-AAD
  • 7-AAD

7-AAD

Cat No. Size Price Quantity
13-6993-T200 200 tests $17.00
13-6993-T500 500 tests $35.00

Description

7-AAD (7-Aminoactinomycin D) is a nucleic acid dye that can be used to exclude nonviable cells from the analysis of flow cytometry data.

 

Recent Publications:

Yukimoto A, Watanabe T, Sunago K, Nakamura Y, Tanaka T, Koizumi Y, Yoshida O, Tokumoto Y, Hirooka M, Abe M, Hiasa Y. The long noncoding RNA of RMRP is downregulated by PERK, which induces apoptosis in hepatocellular carcinoma cells. Sci Rep. 2021 Apr 12;11(1):7926. doi: 10.1038/s41598-021-86592-6. PMID: 33846370; PMCID: PMC8041825.

» View on Nature

Ornell KJ, Chiu B, Coburn JM. Development of a Dinutuximab Delivery System Using Silk Foams for GD2 Targeted Neuroblastoma Cell Death. J Biomed Mater Res A. 2020 Nov 30. doi: 10.1002/jbm.a.37131. PMID: 33252182.

» View on PubMed

Yonebayashi S, Tajiri K, Murakoshi N, Xu D, et al. MAIR-II deficiency ameliorates cardiac remodelling post-myocardial infarction by suppressing TLR9-mediated macrophage activation. J Cell Mol Med. 2020 Nov 2. doi: 10.1111/jcmm.16070. PMID: 33140535.   

» View on PubMed

Matsuo T, Tashiro H, Sumiyoshi R, Saito S, Shirasaki R, Shirafuji N. Functional expression cloning of molecules inducing CD34 expression in bone marrow-derived stromal myofibroblasts. Biochem Biophys Res Commun. 2020 Oct 13:S0006-291X(20)31909-4. doi: 10.1016/j.bbrc.2020.10.006. PMID: 33066959. 

» View on PubMed

Takahashi D, Hoshina N, Kabumoto Y, et al. Microbiota-derived butyrate limits the autoimmune response by promoting the differentiation of follicular regulatory T cells. EBioMedicine. 2020;58:102913. doi:10.1016/j.ebiom.2020.102913.

» View on PubMed

Jonathan J Pinney, Fátima Rivera-Escalera, Charles C Chu, Hannah E Whitehead, Karl VanDerMeid, Ashley M Nelson, Michelle C Barbeau, Clive S Zent, Michael R Elliott; Macrophage hypophagia as a mechanism of innate immune exhaustion in mAb-induced cell clearance. Blood.2020005571. doi: https://doi.org/10.1182/blood.2020005571.

» View on PubMed

Homann S, Hofmann C, Gorin AM, Nguyen HCX, Huynh D, Hamid P, Maithel N, Yacoubian V, Mu W, Kossyvakis A, Sen Roy S, Yang OO and Kelesidis T. 2017. A novel rapid and reproducible flow cytometric method for optimization of transfection efficiency in cells. PLoS One. doi: 10.1371/journal.pone.0182941.

» View on PubMed

Koshy Cherian A, Parikh V, Wu Q, Mao-Draayer Y, Wang Q, Blakely RD and Sarter M. 2017. Hemicholinium-3 sensitive choline transport in human T lymphocytes: Evidence for use as a proxy for brain choline transporter (CHT) capacity. Neurochem Int. doi: 10.1016/j.neuint.2017.05.022.

» View on PubMed

Kamachi F, Isshiki T, Harada N, Akiba H and Miyake S. 2015. ICOS promotes group 2 innate lymphoid cell activation in lungs. Biochem Biophys Res Commun. doi: 10.1016/j.bbrc.2015.06.005.

» View on PubMed

Huang B, Faucette AN, Pawlitz MD, Pei B, Goyert JW, Zhou JZ, El-Hage NG, Deng J, Lin J, Yao F, Dewar III RS, Jassal JS, Sandberg ML, Dai J, Cols M, Shen C, Polin LA, Nichols RA, Jones TB, Bluth MH, Puder KS, Gonik B, Nayak NR, Puscheck E, Wei WZ, Cerutti A, Colonna M and Chen K. 2016. Interleukin-33-induced expression of PIBF1 by decidual B cells protects against preterm labor. Nat. Med. doi:10.1038/nm.4244

» View on PubMed

 

View More

7-AAD (7-Aminoactinomycin D) is a nucleic acid dye that can be used to exclude nonviable cells from the analysis of flow cytometry data.

 

Recent Publications:

Yukimoto A, Watanabe T, Sunago K, Nakamura Y, Tanaka T, Koizumi Y, Yoshida O, Tokumoto Y, Hirooka M, Abe M, Hiasa Y. The long noncoding RNA of RMRP is downregulated by PERK, which induces apoptosis in hepatocellular carcinoma cells. Sci Rep. 2021 Apr 12;11(1):7926. doi: 10.1038/s41598-021-86592-6. PMID: 33846370; PMCID: PMC8041825.

» View on Nature

Ornell KJ, Chiu B, Coburn JM. Development of a Dinutuximab Delivery System Using Silk Foams for GD2 Targeted Neuroblastoma Cell Death. J Biomed Mater Res A. 2020 Nov 30. doi: 10.1002/jbm.a.37131. PMID: 33252182.

» View on PubMed

Yonebayashi S, Tajiri K, Murakoshi N, Xu D, et al. MAIR-II deficiency ameliorates cardiac remodelling post-myocardial infarction by suppressing TLR9-mediated macrophage activation. J Cell Mol Med. 2020 Nov 2. doi: 10.1111/jcmm.16070. PMID: 33140535.   

» View on PubMed

Matsuo T, Tashiro H, Sumiyoshi R, Saito S, Shirasaki R, Shirafuji N. Functional expression cloning of molecules inducing CD34 expression in bone marrow-derived stromal myofibroblasts. Biochem Biophys Res Commun. 2020 Oct 13:S0006-291X(20)31909-4. doi: 10.1016/j.bbrc.2020.10.006. PMID: 33066959. 

» View on PubMed

Takahashi D, Hoshina N, Kabumoto Y, et al. Microbiota-derived butyrate limits the autoimmune response by promoting the differentiation of follicular regulatory T cells. EBioMedicine. 2020;58:102913. doi:10.1016/j.ebiom.2020.102913.

» View on PubMed

Jonathan J Pinney, Fátima Rivera-Escalera, Charles C Chu, Hannah E Whitehead, Karl VanDerMeid, Ashley M Nelson, Michelle C Barbeau, Clive S Zent, Michael R Elliott; Macrophage hypophagia as a mechanism of innate immune exhaustion in mAb-induced cell clearance. Blood.2020005571. doi: https://doi.org/10.1182/blood.2020005571.

» View on PubMed

Homann S, Hofmann C, Gorin AM, Nguyen HCX, Huynh D, Hamid P, Maithel N, Yacoubian V, Mu W, Kossyvakis A, Sen Roy S, Yang OO and Kelesidis T. 2017. A novel rapid and reproducible flow cytometric method for optimization of transfection efficiency in cells. PLoS One. doi: 10.1371/journal.pone.0182941.

» View on PubMed

Koshy Cherian A, Parikh V, Wu Q, Mao-Draayer Y, Wang Q, Blakely RD and Sarter M. 2017. Hemicholinium-3 sensitive choline transport in human T lymphocytes: Evidence for use as a proxy for brain choline transporter (CHT) capacity. Neurochem Int. doi: 10.1016/j.neuint.2017.05.022.

» View on PubMed

Kamachi F, Isshiki T, Harada N, Akiba H and Miyake S. 2015. ICOS promotes group 2 innate lymphoid cell activation in lungs. Biochem Biophys Res Commun. doi: 10.1016/j.bbrc.2015.06.005.

» View on PubMed

Huang B, Faucette AN, Pawlitz MD, Pei B, Goyert JW, Zhou JZ, El-Hage NG, Deng J, Lin J, Yao F, Dewar III RS, Jassal JS, Sandberg ML, Dai J, Cols M, Shen C, Polin LA, Nichols RA, Jones TB, Bluth MH, Puder KS, Gonik B, Nayak NR, Puscheck E, Wei WZ, Cerutti A, Colonna M and Chen K. 2016. Interleukin-33-induced expression of PIBF1 by decidual B cells protects against preterm labor. Nat. Med. doi:10.1038/nm.4244

» View on PubMed

 

Hide

Product Details

Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.