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Ghost Dyes™ and Cell Viability Reagents Don't let dead cells haunt your Flow Cytometry data Tonbo's Ghost Dyes™ & Cell Viability Reagents allow for clear live vs. dead cell discrimination for more precise flow cytometry analysis, and are especially useful for intracellular staining protocols. Ghost Dyes™ bind irreversibly to amine groups and are resistant to subsequent washing, fixation and permeabilization. Dead cells with compromised membranes allow Ghost Dyes™ to permeate and bind amine groups of intracellular proteins resulting in fluorescence much brighter than live cells which are impermeant to Ghost Dyes™

Choose from among the following Ghost Dyes™ and Cell Viability Dyes:

Product Name Excitation Laser Emission (nm) Size Cat. No. Price
Ghost Dye™ UV 450 355 405 100 tests 13-0868-T100 $44
Ghost Dye™ UV 450 355 405 500 tests 13-0868-T500 $170
Ghost Dye™ Violet 450 405 450 100 tests 13-0863-T100 $44
Ghost Dye™ Violet 450 405 450 500 tests 13-0863-T500 $170
Ghost Dye™ Violet 510 405 510 100 tests 13-0870-T100 $44
Ghost Dye™ Violet 510 405 510 500 tests 13-0870-T500 $170
Ghost Dye™ Violet 540 405 537 100 tests 13-0879-T100 $44
Ghost Dye™ Violet 540 405 537 500 tests 13-0879-T500 $170
Ghost Dye™ Red 710 633 710 100 tests 13-0871-T100 $44
Ghost Dye™ Red 710 633 710 500 tests 13-0871-T500 $170
Ghost Dye™ Red 780 633 780 100 tests 13-0865-T100 $44
Ghost Dye™ Red 780 633 780 500 tests 13-0865-T500 $170
Propidium Iodide 488, 532, 561 617 200 tests 13-6990-T200 $29
Propidium Iodide 488, 532, 561 617 500 tests 13-6990-T500 $57
7-AAD 488, 532, 561 647 200 tests 13-6993-T200 $17
7-AAD 488, 532, 561 647 500 tests 13-6993-T500 $35

Ghost Dyes™ Protocol

Other Materials Required

  • 1X PBS (azide-free, protein/serum-free)
  • Flow Cytometry Staining Buffer (Stain Buffer) (1X PBS with 2% FBS, 0.09% Na-Azide)
  1. Remove Ghost Dye vial from freezer and allow to equilibrate to room temperature.
  2. Quick spin Ghost Dye vial before opening.
  3. Prepare azide- and protein/serum-free 1X PBS for labeling procedure.
  4. Wash cells twice in 1-2 mL azide- and protein/serum-free 1X PBS. Spin at 300-400 x g for 5 minutes at room temperature and decant supernatant.
  5. Resuspend cells to a concentration of 1-10 x 106/mL in azide- and protein/serum-free 1X PBS.
  6. Add 1 uL of Ghost Dye solution for each 1 mL of cell suspension and vortex immediately. Note: Ghost Dyes are formulated in DMSO, pipet carefully and slowly.
  7. Incubate for 30 minutes at 2-8°C protected from light.
  8. Wash cells 1-2 times with 1-2 mL Stain Buffer. Washing with a protein containing buffer allows removal of any unreacted dye prior to staining with fluorescent antibodies.
  9. Cells can be subsequently stained, fixed and permeabilized according to user protocol.

Note: Cells labeled with Ghost Dyes can be cryopreserved for later use or used in intracellular staining protocols without any loss of fluorescence intensity.

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