Brefeldin A Solution
Teghanemt A, Pulipati P, Misel-Wuchter K, Day K, Yorek MS, Yi R, Keen HL, Au C, Maretzky T, Gurung P, Littman DR, Issuree PD. CD4 expression in effector T cells depends on DNA demethylation over a developmentally established stimulus-responsive element. Nat Commun. 2022 Mar 18;13(1):1477. doi: 10.1038/s41467-022-28914-4. PMID: 35304452.
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Cell Stimulation Cocktail (500X)
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Flow Cytometry Perm Buffer (10X), TNB-1213
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Kevin C. Osum, Adam L. Burrack, Tijana Martinov, Nathanael L. Sahli, Jason S. Mitchell, Christopher G. Tucker, Kristen E. Pauken, Klearchos Papas, Balamurugan Appakalai, Justin A. Spanier, & Brian T. Fife. Interferon-gamma drives programmed death-ligand 1 expression on islet β cells to limit T cell function during autoimmune diabetes. Sci Rep. 2018 May 29;8(1):8295. doi: 10.1038/s41598-018-26471-9
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Foxp3 / Transcription Factor Staining Buffer Kit, TNB-0607
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Chait M, Yilmaz MM, Shakil S, Ku AW, Dogra P, Connors TJ, Szabo PA, Gray JI, Wells SB, Kubota M, Matsumoto R, Poon MM, Snyder ME, Baldwin MR, Sims PA, Saqi A, Farber DL, Weisberg SP. Immune and epithelial determinants of age-related risk and alveolar injury in fatal COVID-19. JCI Insight. 2022 Apr 21:e157608. doi: 10.1172/jci.insight.157608. Epub ahead of print. PMID: 35446789.
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Tsukimoto S, Hakata Y, Tsuji-Kawahara S, Enya T, Tsukamoto T, Mizuno S, Takahashi S, Nakao S, Miyazawa M. Distinctive High Expression of Antiretroviral APOBEC3 Protein in Mouse Germinal Center B Cells. Viruses. 2022 Apr 17;14(4):832. doi: 10.3390/v14040832. PMID: 35458563
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Burrack AL, Schmiechen ZC, Patterson MT, Miller EA, Spartz EJ, Rollins MR, Raynor JF, Mitchell JS, Kaisho T, Fife BT, Stromnes IM. Distinct myeloid antigen-presenting cells dictate differential fates of tumor-specific CD8+ T cells in pancreatic cancer. JCI Insight. 2022 Apr 8;7(7):e151593. doi: 10.1172/jci.insight.151593. PMID: 35393950.
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Teghanemt A, Pulipati P, Misel-Wuchter K, Day K, Yorek MS, Yi R, Keen HL, Au C, Maretzky T, Gurung P, Littman DR, Issuree PD. CD4 expression in effector T cells depends on DNA demethylation over a developmentally established stimulus-responsive element. Nat Commun. 2022 Mar 18;13(1):1477. doi: 10.1038/s41467-022-28914-4. PMID: 35304452.
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Poon MML, Rybkina K, Kato Y, Kubota M, Matsumoto R, Bloom NI, Zhang Z, Hastie KM, Grifoni A, Weiskopf D, Wells SB, Ural BB, Lam N, Szabo PA, Dogra P, Lee YS, Gray JI, Bradley MC, Brusko MA, Brusko TM, Saphire EO, Connors TJ, Sette A, Crotty S, Farber DL. SARS-CoV-2 infection generates tissue-localized immunological memory in humans. Sci Immunol. 2021 Oct 7:eabl9105. doi: 10.1126/sciimmunol.abl9105. PMID: 34618554.
Yoast RE, Emrich SM, Zhang X, Xin P, Arige V, Pathak T, Benson JC, Johnson MT, Abdelnaby AE, Lakomski N, Hempel N, Han JM, Dupont G, Yule DI, Sneyd J, Trebak M. The Mitochondrial Ca2+ Uniporter is a Central Regulator of Interorganellar Ca2+ Transfer and NFAT Activation. J Biol Chem. 2021 Sep 6:101174. doi: 10.1016/j.jbc.2021.101174. PMID: 34499925.
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Davenport BJ, Morrison TE, Kedl RM, Klarquist J. Conserved and Novel Mouse CD8 T Cell Epitopes within SARS-CoV-2 Spike Receptor Binding Domain Protein Identified following Subunit Vaccination. J Immunol. 2021 May 10:ji2100195. doi: 10.4049/jimmunol.2100195. PMID: 33972373.
Kim DI, Cho YB, Lim Y, Hong SH, Hahm B, Lee SM, Kang SC, Seo YJ. Chios mastic gum inhibits influenza A virus replication and viral pathogenicity. J Gen Virol. 2021 Jan 8. doi: 10.1099/jgv.0.001550. PMID: 33416468.
Kumar P, Arbieva ZH, Maienschein-Cline M, Ganesh BB, Ramasamy S, Prabhakar BS. Induction of Antigen-Independent Proliferation of Regulatory T-Cells by TNF Superfamily Ligands OX40L and GITRL. Methods Mol Biol. 2021;2248:63-71. doi: 10.1007/978-1-0716-1130-2_4. PMID: 33185867.
Mishra S, Liao W, Liu Y, Yang M, Ma C, Wu H, Zhao M, Zhang X, Qiu Y, Lu Q, Zhang N. TGF-β and Eomes control the homeostasis of CD8+ regulatory T cells. J Exp Med. 2021 Jan 4;218(1):e20200030. doi: 10.1084/jem.20200030. PMID: 32991667; PMCID: PMC7527976.
Yashiro T, Yura S, Tobita A, Toyoda Y, Kasakura K, Nishiyama C. Pterostilbene reduces colonic inflammation by suppressing dendritic cell activation and promoting regulatory T cell development. FASEB J. 2020 Sep 22. doi: 10.1096/fj.202001502R. PMID: 32964554.
Semionatto IF, Palameta S, Toscaro JM, Manrique-Rincón AJ, Ruas LP, Paes Leme AF, Bajgelman MC. Extracellular vesicles produced by immunomodulatory cells harboring OX40 ligand and 4-1BB ligand enhance antitumor immunity. Sci Rep. 2020 Sep 16;10(1):15160. doi: 10.1038/s41598-020-72122-3. PMID: 32939048.
Poudel B, Yorek MS, Mazgaeen L, Brown SA, Kanneganti TD, Gurung P. Acute IL-4 Governs Pathogenic T Cell Responses during Leishmania major Infection. Immunohorizons. 2020 Sep 18;4(9):546-560. doi: 10.4049/immunohorizons.2000076. PMID: 32948646.
Yagi Y, Kuwahara M, Suzuki J, Imai Y, Yamashita M. Glycolysis and subsequent mevalonate biosynthesis play an important role in Th2 cell differentiation. 2020 Aug 12. Biochem Biophys Res Commun. 2020;S0006-291X(20)31554-0. doi:10.1016/j.bbrc.2020.08.009.
Conor N. Gruber, Jorg J.A. Calis, Sofija Buta, et al. Complex Autoinflammatory Syndrome Unveils Fundamental Principles of JAK1 Kinase Transcriptional and Biochemical Function. Immunity, 2020 Aug 3. https://doi.org/10.1016/j.immuni.2020.07.006
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J. Michael Stolley, Timothy S. Johnston, Andrew G. Soerens, Lalit K. Beura, Pamela C. Rosato, Vineet Joag, Sathi P. Wijeyesinghe, Ryan A. Langlois, Kevin C. Osum, Jason S. Mitchell, David Masopust; Retrograde migration supplies resident memory T cells to lung-draining LN after influenza infection. J Exp Med3 June 2020; 217 (8): e20192197. doi: https://doi.org/10.1084/jem.20192197.
IL-6 Deficiency Exacerbates Allergic Asthma and Abrogates the Protective Effect of Allergic Inflammation against Streptococcus pneumoniae Pathogenesis. Taylor Schmit, Sumit Ghosh, Ram Kumar Mathur, Tyler Barnhardt, Ganesh Ambigapathy, Min Wu, Colin Combs, M. Nadeem Khan. The Journal of Immunology June 15, 2020, ji1900755; DOI: 10.4049/jimmunol.1900755.
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Correlation of the levels of DNA-binding inhibitor Id3 and regulatory T cells with SLE disease severity. ChenLiu. SenYu. RongJin. YanLong. SongsongLu. YingSong. XiuyuanSun. Xiao-HongSun. YuZhang. J Autoimmun. 2020 Jun 11;102498. doi: 10.1016/j.jaut.2020.102498.
Franco da Cunha F, Andrade-Oliveira V, Candido de Almeida D, Borges da Silva T, Naffah de Souza Breda C, Costa Cruz M, Faquim-Mauro EL, Antonio Cenedeze M, Ioshie Hiyane M, Pacheco-Silva A, Aparecida Cavinato R, Torrecilhas AC, Olsen Saraiva Câmara N. Extracellular Vesicles isolated from Mesenchymal Stromal Cells Modulate CD4+ T Lymphocytes Toward a Regulatory Profile. Cells. 2020 Apr 23;9(4). pii: E1059. doi: 10.3390/cells9041059Extracellular Vesicles isolated from Mesenchymal Stromal Cells Modulate CD4+ T Lymphocytes Toward a Regulatory Profile. Cells. 2020 Apr 23;9(4). pii: E1059. doi: 10.3390/cells9041059
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Adam L. Burrack, Ellen J. Spartz, Jackson F. Raynor, Iris Wang, Margaret Olson, Ingunn M. Stromnes. Combination PD-1 and PD-L1 Blockade Promotes Durable Neoantigen-Specific T Cell-Mediated Immunity in Pancreatic Ductal Adenocarcinoma. Cell Rep. 2019 Aug 20;28(8):2140-2155.e6. doi: 10.1016/j.celrep.2019.07.059
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Laura S. Angelo, Lynn H. Bimler, Rana Nikzad, Kevin Aviles-Padilla, and Silke Paust. CXCR6+ NK Cells in Human Fetal Liver and Spleen Possess Unique Phenotypic and Functional Capabilities. Front Immunol. 2019 Mar 19;10:469. doi: 10.3389/fimmu.2019.00469
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Foxp3 / Transcription Factor Fix/Perm Concentrate (4X), TNB-1020
Xu K, Li MO. Delivery of membrane impermeable molecules to primary mouse T lymphocytes. STAR Protoc. 2021 Aug 17;2(3):100757. doi: 10.1016/j.xpro.2021.100757. PMID: 34458872; PMCID: PMC8377589.
Pingili AK, Chaib M, Sipe LM, et al. Immune checkpoint blockade reprograms systemic immune landscape and tumor microenvironment in obesity-associated breast cancer. Cell Rep. 2021 Jun 22;35(12):109285. doi: 10.1016/j.celrep.2021.109285. PMID: 34161764.
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RBC Lysis Buffer
Picarda E, Galbo PM Jr, Zong H, Rajan MR, Wallenius V, Zheng D, Börgeson E, Singh R, Pessin J, Zang X. The immune checkpoint B7-H3 (CD276) regulates adipocyte progenitor metabolism and obesity development. Sci Adv. 2022 Apr 29;8(17):eabm7012. doi: 10.1126/sciadv.abm7012. Epub 2022 Apr 27. PMID: 35476450; PMCID: PMC9045715.
Zhou X, Fang D, Liu H, Ou X, Zhang C, Zhao Z, Zhao S, Peng J, Cai S, He Y, Xu J. PMN-MDSCs accumulation induced by CXCL1 promotes CD8+ T cells exhaustion in gastric cancer. Cancer Lett. 2022 Feb 14:215598. doi: 10.1016/j.canlet.2022.215598. Epub ahead of print. PMID: 35176418.
Young IC, Wu B, Andricovich J, Chuang ST, Li R, Tzatsos A, Wu RC, Wu MY. Differentiation of fetal hematopoietic stem cells requires ARID4B to restrict autocrine KITLG/KIT-Src signaling. Cell Rep. 2021 Nov 23;37(8):110036. doi: 10.1016/j.celrep.2021.110036. PMID: 34818550.
Fan Z, Jan S, Hickey JC, Davies DH, Felgner J, Felgner PL, Guan Z. Multifunctional Dendronized Polypeptides for Controlled Adjuvanticity. Biomacromolecules. 2021 Nov 17. doi: 10.1021/acs.biomac.1c01052. Epub ahead of print. PMID: 34788023.
Li X, Liu J, Xing Z, Tang J, Sun H, Zhang X, Lv S, Chen Z, Shi M, Chen M, Zuo S, Lyu X, He Y. Polymorphonuclear myeloid-derived suppressor cells link inflammation and damage response after trauma. J Leukoc Biol. 2021 Oct 12. doi: 10.1002/JLB.3MA0821-029R. Epub ahead of print. PMID: 34636072.
Prosser A, Dart S, Larma-Cornwall I, Lucas M. Flow cytometric characterization of tissue-resident lymphocytes after murine liver and heart transplantation. STAR Protoc. 2021 Sep 15;2(4):100810. doi: 10.1016/j.xpro.2021.100810. PMID: 34568841; PMCID: PMC8449134.
Mi L, Wang Y, Xu H, Wang Y, Wu J, Dai H, Zhang Y. PRAK Promotes the Pathogen Clearance by Macrophage Through Regulating Autophagy and Inflammasome Activation. Front Immunol. 2021 Apr 16;12:618561. doi: 10.3389/fimmu.2021.618561. PMID: 33936034; PMCID: PMC8085562.
Trott DW, Islam MT, Buckley DJ, Donato AJ, Dutson T, Sorensen ES, Cai J, Gogulamudi VR, Phuong TTT, Lesniewski LA. T lymphocyte depletion ameliorates age-related metabolic impairments in mice. Geroscience. 2021 Apr 24. doi: 10.1007/s11357-021-00368-4. PMID: 33893902.
Bake S, Pinson MR, Pandey S, et al. Prenatal Alcohol-Induced Sex Differences in Immune, Metabolic and Neurobehavioral Outcomes in Adult Rats. Brain Behav Immun. 2021 Aug 11:S0889-1591(21)00497-9. doi: 10.1016/j.bbi.2021.08.207. PMID: 34390803.
Mi L, Wang Y, Xu H, Wang Y, Wu J, Dai H, Zhang Y. PRAK Promotes the Pathogen Clearance by Macrophage Through Regulating Autophagy and Inflammasome Activation. Front Immunol. 2021 Apr 16;12:618561. doi: 10.3389/fimmu.2021.618561. PMID: 33936034; PMCID: PMC8085562.
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Jennifer S. Chen, Jessica D.S. Grassmann, Uthaman Gowthaman, Sam J. Olyha, Tregony Simoneau, M. Cecilia Berin, Stephanie C. Eisenbarth, Adam Williams. Flow cytometric identification of Tfh13 cells in mouse and human. Journal of Allergy and Clinical Immunology, 2020. https://doi.org/10.1016/j.jaci.2020.04.063.
Jackson LP, Tjoa BA, Mellert H, Pestano GA. Development of a TCR beta repertoire assay for profiling liquid biopsies from NSCLC donors. Cancer Drug Resist 2020;3. http://dx.doi.org/10.20517/cdr.2020.07.
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Flow Staining Buffer (1X)
Burbach BJ, O'Flanagan SD, Shao Q, et al. Irreversible electroporation augments checkpoint immunotherapy in prostate cancer and promotes tumor antigen-specific tissue-resident memory CD8+ T cells. Nat Commun. 2021 Jun 23;12(1):3862. doi: 10.1038/s41467-021-24132-6. PMID: 34162858.
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Pingili AK, Chaib M, Sipe LM, et al. Immune checkpoint blockade reprograms systemic immune landscape and tumor microenvironment in obesity-associated breast cancer. Cell Rep. 2021 Jun 22;35(12):109285. doi: 10.1016/j.celrep.2021.109285. PMID: 34161764.
Monensin Solution (1000X)
Dodagatta-Marri E, Ma HY, Liang B, et al. Integrin αvβ8 on T cells suppresses anti-tumor immunity in multiple models and is a promising target for tumor immunotherapy. Cell Rep. 2021 Jul 6;36(1):109309. doi: 10.1016/j.celrep.2021.109309. PMID: 34233193.
Foxp3 / Transcription Factor Fix/Perm Diluent (1X), TNB-1022
Xu K, Li MO. Delivery of membrane impermeable molecules to primary mouse T lymphocytes. STAR Protoc. 2021 Aug 17;2(3):100757. doi: 10.1016/j.xpro.2021.100757. PMID: 34458872; PMCID: PMC8377589.