Gibson Assembly® Site-Directed Mutagenesis
The Gibson Assembly® Ultra Kit utilizes a robust two-step method to seamlessly join 1 to 15 inserts into a plasmid vector. The entire process can be completed in 80 minutes or less. Unlike other approaches, SGI-DNA’s Gibson Assembly® process utilizes Phusion® High-Fidelity DNA polymerase for 3’ end extension. This proof-reading process results in higher numbers of seamless error-free clones ready for multiple downstream applications.
Using Gibson Assembly® Ultra method, multiple DNA fragments from 100 bp to 100 kb can be joined with 95% cloning efficiency and constructs up to 1000 kb in length can be efficiently assembled by designing multi-stage Gibson Assembly® Ultra reactions.Gibson Assembly Ultra methodology is available in the following formats:
• Kit (which includes a positive control) or Master Mix (only)
• 5, 10, or 50 reaction sizes