Gibson Assembly®
Tonbo offers several Gibson Assembly® cloning kits for DNA assembly. Each kit contains specific master mix reagents and proof-reading polymerase.
Gibson Assembly® HiFi 1-Step Kit 
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Gibson Assembly® Ultra Kit  |
Gibson Assembly® Site-Directed Mutagenesis Kit  |
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|---|---|---|---|
| Hands-on time | <5 min | <6 min | <40 min |
| Total reaction time | 60 min | 80 min | ~4-6 hr |
| Number of steps | 1 | 2 | 3 |
| Fragment size range | 500 bp to 32 kb | 100 bp to 100 kb | 500 bp to 5.5 kb |
| Cloning efficiency | >90% | >95% | 95% single site |
| Fragments per reaction | up to 5 | up to 15 | up to 5 |
| Maximum construct size (multiple-stage reactions) | 100 kb | 1000 kb | 25 kb |
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