Ghost Dyes™ & Cell Viability Reagents
Tonbo's Ghost Dyes™ & Cell Viability Reagents allow for clear live vs. dead cell discrimination for more precise flow cytometry analysis, and are especially useful for intracellular staining protocols.
Ghost Dyes™ bind irreversibly to amine groups and are resistant to subsequent washing, fixation and permeabilization. Dead cells with compromised membranes allow Ghost Dyes™ to permeate and bind amine groups of intracellular proteins resulting in fluorescence much brighter than live cells which are impermeant to Ghost Dyes™
I recently obtained an aliquot of Ghost Dye 510 from my core flow lab. I titrated the reagent against a mixture of heat-killed and untreated PBMC. Spooooky Bright! I will be replacing my current Live/Dead indicator with the Ghost Dye 510.
Cindy L. Zuleger, Ph.D.
University of Wisconsin
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